Journal of Biophotonics,

Quantitative assessment of microenvironment characteristics and metabolic activity in glioma via multiphoton microscopy

Abstract Tumor microenvironment and metabolic activity in gliomas are the important biomarkers to evaluate the progression of gliomas. Many evidences have suggested that the targeting of metabolic activity and tumor microenvironment simultaneously can be more effective to take the tumor therapy. Therefore, the non‐invasive, accurate assessment of tumor microenvironment and metabolic activity is quite important in clinical practice. Multiphoton microscopy (MPM), based on two‐photon excited fluorescence...

Journal of Biophotonics

11m

Detection of Mitochondrial Dysfunction in vitro by Laser Induced Autofluorescence

ABSTRACT Mitochondrion plays a significant role in a variety of biological functions. Because of their diverse character and location in the cellular systems, mitochondria commonly get exposed to various extrinsic and intrinsic cellular stresses. The present study reports a novel approach to detection of mitochondrial dysfunction based on tryptophan autofluorescence of its proteins in mouse liver, using Laser Induced Fluorescence (LIF) as a tool. Mitochondria, isolated from the mouse liver, were...

Journal of Biophotonics

11m

Two ways to inactivate the Ki‐67 Protein ‐ Fragmentation by Nanoparticles, Crosslinking with Fluorescent Dyes

Abstract Light can manipulate molecular biological processes with high spatial and temporal precision and optical manipulation has become increasingly popular during the last years. In combination with absorbing dyes or gold nanoparticles light is a valuable tool for cell and protein inactivation with high precision. Here we show distinct differences in the underlying mechanisms whether gold nanoparticles or fluorescent dyes are used for the inactivation of the Ki‐67 protein. The proliferation‐associated...

Journal of Biophotonics

16m

Assessing the development and treatment of rheumatoid arthritis using multi‐parametric photoacoustic and ultrasound imaging

Abstract Rheumatoid arthritis (RA), characterized by polyarthritis, is a chronic, systemic, and inflammatory autoimmune disease. In this study, we developed a dual‐modality multi‐parametric photoacoustic and ultrasound imaging technique, and successfully derived multiple parameters such as relative concentration of total hemoglobin (CHbT), ratio of angiogenesis, joint size and area of synovia to assess the development and treatment of RA. We established a model of adjuvant arthritis using a total...

Journal of Biophotonics

16m

Monte Carlo Modelling of Photon Migration in Realistic Human Thoracic Tissues for Noninvasive Monitoring of Cardiac Hemodynamics

Noninvasive monitoring of cardiac hemodynamics remains challenging in cardiovascular medicine. The possibility of noninvasive optical monitoring of cardiac hemodynamics was theoretically investigated in this study. By utilizing the Monte Carlo simulation method for voxelized media (MCVM) and Visible Chinese Human dataset, we quantified and visualized the photon migration in human thoracic region. The light fluence distribution was shown to reach heart tissue (~3cm depth under body surface) and 12%...

Journal of Biophotonics

16m

Determination of Optical Parameters of the Porcine Eye and Development of a Simulated Model

The eye is a very sophisticated system of optical elements for the preeminent sense of vision. In recent years, the number of laser surgery to correct the optical aberration such as myopia or astigmatism has significantly increased. Consequently, improving the knowledge related to the interactions of light with the eye is very important in order to enhance the efficiency of the surgery. For this reason, a complete optical characterization of the porcine eye is presented in this study. Kubelka‐Munk...

Journal of Biophotonics

16m