Iron Deficiency Is Associated with Maternal Hypothyroxinemia in the Third Trimester of Pregnancy

xlomafota13 shared this article with you from Inoreader Iron Deficiency Is Associated with Maternal Hypothyroxinemia in the Third Trimester of Pregnancy Via Clinical Thyroidology Clinical Thyroidology, Volume 33, Issue 12, Page 526-528, December 2021. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Asthmatic Patients Have an Increased Risk of Hyperthyroidism

xlomafota13 shared this article with you from Inoreader Asthmatic Patients Have an Increased Risk of Hyperthyroidism Via Clinical Thyroidology Clinical Thyroidology, Volume 33, Issue 12, Page 523-525, December 2021. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Obesity Is Associated with Increased Adipocyte Infiltration in the Thyroid Tissue: A New Perspective?

xlomafota13 shared this article with you from Inoreader Obesity Is Associated with Increased Adipocyte Infiltration in the Thyroid Tissue: A New Perspective? Via Clinical Thyroidology Clinical Thyroidology, Volume 33, Issue 12, Page 516-519, December 2021. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

The Learning Curve for Radiofrequency Ablation of Benign Thyroid Nodules

xlomafota13 shared this article with you from Inoreader The Learning Curve for Radiofrequency Ablation of Benign Thyroid Nodules Via Clinical Thyroidology Clinical Thyroidology, Volume 33, Issue 12, Page 529-531, December 2021. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Identification of potential biomarkers for diagnosis of hepatocellular carcinoma

xlomafota13 shared this article with you from Inoreader Identification of potential biomarkers for diagnosis of hepatocellular carcinoma Via Experimental and therapeutic medicine Exp Ther Med. 2022 Jan;23(1):51. doi: 10.3892/etm.2021.10973. Epub 2021 Nov 15. ABSTRACT Hepatocellular carcinoma (HCC) has a high mortality rate owing to its complexity. Identification of abnormally expressed genes in HCC tissues compared to those in normal liver tissues is a viable strategy for investigating the mechanisms of HCC tumorigenesis and progression as a means of developing novel treatments. A significant advantage of the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) is that the data therein were collected from different independent researchers and may be integrated, allowing for a more robust data analysis. Accordingly, in the present study, the gene expression profiles for HCC and control samples were downloaded from the GEO and TCGA. Functional enrichment analysis was performed using a Metascape dataset, and a protein-protein interaction (PPI) network was constructed using the Search Tool for the Retri eval of Interacting Genes/proteins (STRING) online database. The prognostic value of mRNA for HCC was assessed using the Kaplan-Meier Plotter, a public online tool. A gene mRNA heatmap and DNA amplification numbers were obtained from cBioPortal. A total of 2,553 upregulated genes were identified. Functional enrichment analysis revealed that these differentially expressed genes (DEGs) were mainly accumulated in metabolism of RNA and the cell cycle. Considering the complexity and heterogeneity of the molecular alterations in HCC, multiple genes for the prognostication of patients with HCC are more reliable than a single gene. Thus, the PPI network and univariate Cox regression analysis were applied to screen candidate genes (small nuclear ribonucleoprotein polypeptide B and B1, nucleoporin 37, Rac GTPase activating protein 1, kinesin family member 20A, minichromosome maintenance 10 replication initiation factor, ubiquitin conjugating enzyme E2 C and hyaluronan mediated motility recept or) that are associated with the overall survival and progression-free survival of patients with HCC. In conclusion, the present study identified a set of genes that are associated with overall survival and progression-free survival of patients with HCC, providing valuable information for the prognosis of HCC. PMID:34917180 | PMC:PMC8630445 | DOI:10.3892/etm.2021.109 73 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Impact of modern personalized treatment of breast cancer on surgical attitude and outcomes

xlomafota13 shared this article with you from Inoreader Impact of modern personalized treatment of breast cancer on surgical attitude and outcomes Via Experimental and therapeutic medicine Exp Ther Med. 2022 Jan;23(1):57. doi: 10.3892/etm.2021.10979. Epub 2021 Nov 17. ABSTRACT Multimodal treatment of breast cancer has made steady progress in recent years. The involvement of modern oncology, diagnostic imaging techniques and surgical treatment, have brought a definite benefit to patients, defining the multidisciplinary treatment of breast cancer. The introduction of immunohistochemical testing and genetic screening has led to the prioritization of therapy according to their results and a correct approach to initiating treatment. The main aim of the present study was to conduct a comparative analysis through a retrospective study of the therapeutic means used in breast cancer with the statistical evaluation of the obtained results. To carry out the study, a group of 125 patients hospitalized during the period January 2015 to December 2020, were included, and the parameters were selected from the observation sheets. The res ults of the study demonstrated the superiority of multimodal treatment of breast cancer over surgical treatment as the only therapeutic management. The introduction of ultrasound-guided biopsies and conservative surgical options has led to increased diagnostic accuracy and a significant improvement in aesthetic outcome. The multidisciplinary approach to breast cancer allows an individualized treatment by performing immunohistochemical testing and through the use of neoadjuvant and adjuvant treatment combined with conservative surgical techniques with a more favorable cosmetic and oncological result, with reduced postoperative complications. PMID:34917183 | PMC:PMC8630438 | DOI:10.3892/etm.2021.10979 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

HIF-1α mediates the protective effect of plasma extracellular particles induced by remote ischaemic preconditioning on oxidative stress injury in human umbilical vein endothelial cells

xlomafota13 shared this article with you from Inoreader HIF-1α mediates the protective effect of plasma extracellular particles induced by remote ischaemic preconditioning on oxidative stress injury in human umbilical vein endothelial cells Via Experimental and therapeutic medicine Exp Ther Med. 2022 Jan;23(1):48. doi: 10.3892/etm.2021.10970. Epub 2021 Nov 15. ABSTRACT Remote ischaemic preconditioning (RIPC) is considered to alleviate myocardial ischaemia/reperfusion (I/R) injury. The present study explored whether blood plasma particulate matter, which is termed extracellular particles (EPs), and is released from cells during RIPC, could reduce H2O2-induced damage in human umbilical vein endothelial cells (HUVECs). Firstly, EPs were derived from volunteers who did or did not undergo RIPC. To induce RIPC in volunteers, a blood pressure cuff was alternatively inflated for 5 min and deflated for the same duration for four successive cycles. HUVECs were assigned to two groups: i) Group 1 was preincubated for 24 h with EPs from volunteers after sham-RIPC, then treated with H2O2 (1 mM; 6 h) to mimic the in vivo conditions of I/R-induced oxidative stress; and ii) group 2 was preincubated for 24 h with EPs from volunteers after RIPC, then treated with H2O2. Subsequently, EPs were derived from rats received sham-RIPC or RIPC and/or cadmium (Cd) pre-treatment. To induce RIPC in rats, a remote hind limb preconditioning stimulus was delivered using a blood pressure cuff attached at the inguinal level of the rat. The blood pressure cuff was alternatively inflated for 5 min and deflated for the same time period for four successive cycles. HUVECs were assigned to six groups: i) Group 1 was untreated; ii) group 2 received only H2O2 treatment (1 mM; 6 h); iii) group 3 was preincubated for 24 h with EPs from rats exposed to sham-RIPC, then treated with H2O2; iv) group 4 was preincubated for 24 h with EPs from rats that received an intraperitoneal injection of 1 mg/kg Cd [a pharmacological inhibitor of hypoxia-inducible factor 1-α (HIF-1α) in vivo] 180 min before sham-RIPC, then treat ed with H2O2; v) group 5 was preincubated for 24 h with EPs from rats exposed to RIPC, then treated with H2O2; and vi) group 6 was preincubated for 24 h with EPs from rats that received an intraperitoneal injection of 1 mg/kg Cd 180 min before RIPC, then treated with H2O2. Cell viability and cytotoxicity were monitored using Cell Counting Kit-8 and lactate dehydrogenase assays. Cell apoptosis and necrosis were assessed via flow cytometry and western blot analysis. A notable increase in EP concentration in the plasma of volunteers after RIPC compared with that in the plasma of volunteers after sham-RIPC was observed. RIPC-associated EPs (RIPC-EPs) from volunteers could improve cell viability and reduce cytotoxicity, cell apoptosis and necrosis in HUVECs treated with H2O2 in vitro. Furthermore, RIPC caused a significant increase in HIF-1α expression in the rat limb musculature. The apoptosi s-reducing effect of RIPC-EPs was demonstrated to be counteracted by an intraperitoneal injection of Cd before RIPC in rats. A significant decrease in the EP levels precipitated from the plasma of rats that received Cd treatment before RIPC was observed compared with rats that did not receive Cd treatment. The present study suggested that HIF-1α mediated at least partly the protective effect of plasma RIPC-EPs on oxidative stress injury in HUVECs. PMID:34917179 | PMC:PMC8630 441 | DOI:10.3892/etm.2021.10970 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

ASA Physical Status Classification and Complications Following Facial Fracture Repair

xlomafota13 shared this article with you from Inoreader ASA Physical Status Classification and Complications Following Facial Fracture Repair Via otol rhinol Ann Otol Rhinol Laryngol. 2021 Dec 17:34894211059599. doi: 10.1177/00034894211059599. Online ahead of print. ABSTRACT BACKGROUND: To investigate the association between American Society of Anesthesiologists (ASA) physical status classification and rates of postoperative complications in patients undergoing facial fracture repair. METHODS: Patients were divided into 2 cohorts based on the ASA classification system: Class I/II and Class III/IV. Chi-square and Fisher's ex act tests were used for univariate analyses. Multivariate logistic regressions were used to assess the independent associations of covariates on postoperative complication rates. RESULTS: A total of 3575 patients who underwent facial fracture repair with known ASA classification were identified. Class III/IV patients had higher rates of deep surgical site infection (P = .012) as well as bleeding, readmission, reoperation, surgical, medical, and overall postoperative complications (P < .001). Multivariate regression analysis found that Class III/IV was significantly associated with increased length of stay (P < .001) and risk of overall complications (P = .032). Specifically, ASA Class III/IV was associated with increased rates of deep surgical site infection (P = .049), postoperative bleeding (P = .036), and failure to wean off ventilator (P = .027). CONCLUSIONS: Higher ASA class is associated with increased length o f hospital stay and odds of deep surgical site infection, bleeding, and failure to wean off of ventilator following facial fracture repair. Surgeons should be aware of the increased risk for postoperative complications when performing facial fracture repair in patients with high ASA classification. PMID:34918565 | DOI:10.1177/00034894211059599 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Impact of the Mutational Landscape of the Sodium/Iodide Symporter in Congenital Hypothyroidism

xlomafota13 shared this article with you from Inoreader Impact of the Mutational Landscape of the Sodium/Iodide Symporter in Congenital Hypothyroidism Via liebertpub1 Thyroid, Volume 31, Issue 12, Page 1776-1785, December 2021. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Sinonasal Packing is Not a Requisite for Successful Cerebrospinal Fluid Leak Repair

xlomafota13 shared this article with you from Inoreader Sinonasal Packing is Not a Requisite for Successful Cerebrospinal Fluid Leak Repair Via Journal of Neurological Surgery Part B: Skull Base J Neurol Surg B Skull Base DOI: 10.1055/s-0041-1740622 Background Numerous methods have been described to repair nasal cerebrospinal fluid (CSF) leaks. Most studies have focused on optimizing CSF leak repair success, leading to closure rates of 90 to 95%. Objective This study aimed to determine if excellent reconstruction rates could be achieved without using sinonasal packing. Methods A prospective case series of 73 consecutive patients with various CSF leak etiologies and skull base defects was conducted to evaluate reconstruction success without sinonasal packing. The primary outcome measure was postoperative CSF leak. Secondary outcome measures were postoperative epistaxis requiring intervention in operating room or emergency department, infectious sinusitis, and 22-item sinonasal outcome test (SNOT-22) changes. Results Mean age was 54.5 years and 64% were female. Multilayered reconstructions were performed in 55.3% of cases, with collagen or bone epidural inlay grafts, and nasal mucosal grafts or nasoseptal flaps for onlay layers. Onlay-only reconstructions with mucosal grafts or nasoseptal flaps were performed in 44.7% of cases. Tissue sealants were used in all cases, and lumbar drains were used in 40.8% of cases. There were two initial failures (97.4% initial success), but both resolved with lumbar drains alone (no revision surgeries). There were no instances of postoperative epistaxis requiring intervention in the operating room or emergency department. Infectious sinusitis occurred in 2.7% of patients in the first 3 months postoperatively. SNOT-22 did not change significantly from preoperatively to first postoperative visits, then improved over time. Conclusion Nasal CSF leaks from various etiologies and defect sites were successfully repaired without using sinonasal packing, and patients experienced minimal sinonasal morbidity. [...] Georg Thieme Verlag KG Rüdigerstraße 14, 70469 Stuttgart, Germany Article in Thieme eJournals: Table of contents  |  Abstract  |  Full text View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Distinct Cytokine Signatures in Thyroiditis Induced by PD-1 or CTLA-4 Blockade: Insights from a New Mouse Model

xlomafota13 shared this article with you from Inoreader Distinct Cytokine Signatures in Thyroiditis Induced by PD-1 or CTLA-4 Blockade: Insights from a New Mouse Model Via liebertpub1 Thyroid, Volume 31, Issue 12, Page 1839-1849, December 2021. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Guanylin ligand protects the intestinal immune barrier by activating the guanylate cyclase-C signaling pathway

xlomafota13 shared this article with you from Inoreader Guanylin ligand protects the intestinal immune barrier by activating the guanylate cyclase-C signaling pathway Via histochem Acta Histochem. 2021 Dec 14;124(1):151811. doi: 10.1016/j.acthis.2021.151811. Online ahead of print. ABSTRACT Inflammatory bowel disease (IBD) impacts patient quality of life significantly. The dysfunction of intestinal immune barrier is closely associated with IBD. The guanylate cyclase-C (GC-C) signaling pathway activated by the guanylin (Gn) ligand is involved in the occurrence and development of IBD. However, how it regulates the intestinal immune barrier is still unclea r. To investigate the effect of the GC-C pathway on intestinal mucosal immunity and provide experimental basis for seeking new therapeutic strategies for IBD, we focused on Caco-2 cells and intestinal intra-epithelial lymphocytes (IELs), which displayed inflammatory responses induced by lipopolysaccharide (LPS). GC-C activity was modulated by transfection with Gn overexpression or GC-C shRNA plasmid. Levels of Gn, GC-C, and CFTR; transepithelial electrical resistance (TER); paracellula r permeability; and levels of IL-2, IFN-γ, and secretory IgA (sIgA) were examined. The study found that after stimulation with LPS, Gn, GC-C, CFTR, TER, and sIgA levels were all significantly reduced, IL-2 and IFN-γ levels as well as paracellular permeability were significantly increased. These indicators changed inversely and significantly after transfection with the Gn overexpression vector. Compared to the vector controls, GC-C-silenced cells displayed significantly decreased levels of GC-C, CFTR , and TER and increased levels of IL-2, IFN-γ, and paracellular permeability stimulated by LPS. The results show that Gn ligand can protect the intestinal immune barrier by activating the GC-C signaling pathway, which may be helpful for the development of new treatments for IBD. DATA AVAILABILITY STATEMENT: The data used to support the findings of this study are available from the corresponding author upon request. PMID:34920371 | DOI:10.1016/j.acthis.2021.151811 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.