Paracrine mechanisms of endothelial progenitor cells in vascular repair

xlomafota13 shared this article with you from Inoreader Paracrine mechanisms of endothelial progenitor cells in vascular repair Via histochem Acta Histochem. 2021 Dec 17;124(1):151833. doi: 10.1016/j.acthis.2021.151833. Online ahead of print. ABSTRACT Endothelial progenitor cells (EPCs) play an important role in repairing damaged blood vessels and promoting neovascularization. However, the specific mechanism of EPCs promoting vascular repair is still unclear. Currently, there are two different views on the repair of damaged vessels by EPCs, one is that EPCs can directly differentiate into endothelial cells (ECs) a nd integrate into injured vessels, the other is that EPCs act on cells and blood vessels by releasing paracrine substances. But more evidence now supports the latter. Therefore, the paracrine mechanisms of EPCs are worth further study. This review describes the substances secreted by EPCs, some applications based on paracrine effects of EPCs, and the studies of paracrine mechanisms in cardiovascular diseases--all of these are to support the view that EPCs repair blood vessels through paracrine effects rather than integrating directly into damaged vessels. PMID:34929523 | DOI:10.1016/j.acthis.2021.151833 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Preclinical evidence for pioglitazone and bexarotene combination in oral cancer chemoprevention

xlomafota13 shared this article with you from Inoreader Preclinical evidence for pioglitazone and bexarotene combination in oral cancer chemoprevention Via Head & Neck Abstract Background Head and neck squamous cell carcinoma (HNSCC) requires new treatments and targeted approaches to improve survival. The peroxisome proliferator-activated receptor γ (PPARγ) and retinoic X receptor alpha (RXRα) nuclear receptor pathways may be targetable with repurposed Food and Drug Administration (FDA)-approved agents for prevention and treatment. Methods Oral cancer and leukoplakia cell lines were treated with the PPARγ agonist (pioglitazone) and RXRα activator (bexarotene). PPARγ activation, cellular proliferation, apoptosis activity and phenotype, including the pharmacodynamic marker, involucrin (IVL), were subsequently analyzed using a reporter gene assay, genomic data, MTT assay and western blot. Results Microarray analysis of HNSCC tumor versus normal tissue shows IVL expression is significantly increased in normal tissue compared to HNSCC tumors (p < 0.0001). In MSK Leuk1 and CA 9-22 cell lines, pioglitazone increases PPARγ DNA binding activity and IVL promoter activity in a dose dependent manner (p < 0.01 and p < 0.0001). Combination treatment with pioglitazone and bexarotene increases PPARγ DNA binding activity and IVL promoter activity (p < 0.01 and p < 0.0001). MTT analysis shows decreases in cell proliferation when cells are treated with pioglitazone and bexarotene. Decreases in cell proliferation are significant to at least p < 0.05 for all combination versus single agent treatments. Western blot on whole-cell lysate from cells treated with pioglitazone and bexarotene alone or in combination for IVL showed increased protein levels with combination treatment. Conclusions Targeting the PPARγ/RXRα heterodimer with pioglitazone and bexarotene was effective in this preclinical project. This was functional in both preneoplastic and oral cancer cell lines. A better understanding of the molecular mechanism on downstream effects on cellular proliferation could potentially have implications clinically, both in oral preneoplasia and possibly head and neck cancer; however, more research needs to be done to explore the potential these medications have in chemoprevention. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

An audit of post‐operative pain scores after endoscopic and microscopic ear surgery

xlomafota13 shared this article with you from Inoreader An audit of post‐operative pain scores after endoscopic and microscopic ear surgery Via Clinical Otolaryngology Abstract -Endoscopic ear surgery is often described as minimally invasive, which may reduce postoperative pain -VAS pain scores related to endoscopic ear surgery were lower than those related to microscopic ear surgery in our cohort within the first post-operative week -At day one, two and seven, analgesia requirements were lower in the endoscopic ear surgery group than the microscopic ear surgery group -Opioid analgesia requirement was lower in the endoscopic ear surgery group than the microscopic ear surgery group at days one and two post-operatively -Due to the differing applications of endoscopic and microscopic ear surgery, further research may be required to study the influence of type of surgery, degree of bone removal and approach on pain after ear surgery. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

What is the impact of mastoid surgery on swimming?

xlomafota13 shared this article with you from Inoreader What is the impact of mastoid surgery on swimming? Via Clinical Otolaryngology Abstract Swimming confers significant physical and mental health benefits to people but there is little evidence regarding the impact of mastoid surgery on this activity. Almost half of the patients (46.7%) swim less after mastoid surgery. Occasional swimmers are affected mostly. The need to protect the ear and dizziness are the main reasons to discontinue swimming. Acute incapacitating vertigo occurs in 8.9% patients during swimming. No difference in swimming frequency between patients with a closed or an open mastoid cavity was observed. The presence of a wet ear after surgery and the use of prophylactic measures did not affect the swimming frequency. Patients should not be discouraged from swimming on the basis of mastoid surgery. We recommend a trial of swimming with or without protection in shallow waters first, under the supervision of an experience swimmer. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Efficacy and Safety of External Volume Expansion (EVE) on Fat Grafting: A Systematic Review and Single-Arm Meta-Analysis

xlomafota13 shared this article with you from Inoreader Efficacy and Safety of External Volume Expansion (EVE) on Fat Grafting: A Systematic Review and Single-Arm Meta-Analysis Via Journal of plastic, reconstructive & aesthetic surgery : JPRAS J Plast Reconstr Aesthet Surg. 2021 Nov 16:S1748-6815(21)00576-3. doi: 10.1016/j.bjps.2021.11.032. Online ahead of print. ABSTRACT BACKGROUND: The autologous fat grafting is commonly used for reconstructive or aesthetic purposes. However, due to the huge variation in methods, its retention rate varies a lot. External volume expansion (EVE) has been used to treat recipient sites of fat grafting. Concerns have been raised regarding its efficacy and safety. METHODS: We have searched PubMed, EMBASE, and the Cochrane Library for studies on EVE-assisted fat grafting published from 2000 to 2020. A meta-analysis was conducted to pool the retention rate. The incidence of complications was assessed for reconstructive or aesthetic purposes. RESULTS: The 11 included studies involved 1152 patients with operations on 1794 breasts. Four studies were included in the meta-analysis. The pooled retention rate was 65% [95%CI 49, 79]. Eight stu dies reported the complications. The total complication incidence was 34%, which is 35% for the aesthetic group and 33% for the reconstructive group. The complication rate was not obviously different between the two groups. CONCLUSIONS: The study shows that the EVE-assisted fat grafting has better retention rate than traditional fat grafting. However, the data showed that the complication rate was much higher in the EVE-assisted group. PMID:34930704 | DOI:10.1016/j.bjps.2021.11.032 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Reconstruction of midline abdominal defects with a deep inferior epigastric artery keystone-type perforator flap

xlomafota13 shared this article with you from Inoreader Reconstruction of midline abdominal defects with a deep inferior epigastric artery keystone-type perforator flap Via Journal of plastic, reconstructive & aesthetic surgery : JPRAS J Plast Reconstr Aesthet Surg. 2021 Dec 7:S1748-6815(21)00650-1. doi: 10.1016/j.bjps.2021.11.103. Online ahead of print. NO ABSTRACT PMID:34930700 | DOI:10.1016/j.bjps.2021.11.103 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Filler-induced complications of the lips: 10 years experience with intralesional laser treatment and refinements

xlomafota13 shared this article with you from Inoreader Filler-induced complications of the lips: 10 years experience with intralesional laser treatment and refinements Via Journal of plastic, reconstructive & aesthetic surgery : JPRAS J Plast Reconstr Aesthet Surg. 2021 Nov 15:S1748-6815(21)00586-6. doi: 10.1016/j.bjps.2021.11.042. Online ahead of print. ABSTRACT BACKGROUND: The number of minimally invasive cosmetic procedures has steadily increased every year. In the last decade, the rates of filler injection reactions have also increased. This study presents our experience in the management and treatment of permanent filler complications to the lips. Undoubtedly, in these adverse reactions, the lips are the hallmark that most stigmatizes an unnatural look of the face. METHODS: One hundred and eighty-one patients (168 female and 13 male) with permanent filler complications to the lips were treated from September of 2009 to September of 2019 at the University Hospital of Modena. One hundred and thirty-five patients underwent intralesional laser treatment (ILT). Forty-six patients underwent a stab incision and drainage and 10 of these patients had solid and cal cified nodules and therefore underwent a combined technique. Thirty-three patients underwent further cosmetic procedures. RESULTS: In 95% of patients there was a clinical improvement of which complete resolution in 38.7% and partial improvement in 34.2% of cases. Nine patients stopped the treatment because they were not satisfied. No patient was considered to have worsened after the treatment. Thirty-three patients underwent further treatments. CONCLUSIONS: A systematic combined approach between antibiotic therapy and treatments (ILT and a stab incision) represents the best therapeutic approach in dealing with permanent fillers complications to the lips. This treatment modality allows both the removal of the foreign material and the inflammatory reaction with low morbidity and little cosmetic disfigurement. Further cosmetic procedures may be applied to improve aesthetic appearance once most of the non-resorbable substance has been removed. PMID:34930702 | DOI:10.1016/j.bjps.2021.11.042 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

A retrospective review of transcutaneous and transconjunctival dual approach-A technique for mini-invasive double eyelid blepharoplasty with blepharoptosis correction

xlomafota13 shared this article with you from Inoreader A retrospective review of transcutaneous and transconjunctival dual approach-A technique for mini-invasive double eyelid blepharoplasty with blepharoptosis correction Via Journal of plastic, reconstructive & aesthetic surgery : JPRAS J Plast Reconstr Aesthet Surg. 2021 Nov 28:S1748-6815(21)00609-4. doi: 10.1016/j.bjps.2021.11.062. Online ahead of print. ABSTRACT BACKGROUND: Many Oriental eyes feature single eyelid fold and ptotic eyelid. Performing the double eyelid blepharoplasty (DEB) in a minimally invasive manner with simultaneous blepharoptosis correction (BPC) is important to achieve an aesthetic pleasing outcome. OBJECTIVE: To demonstrate an effective mini-invasive transcutaneous and transconjunctival dual approach technique for simultaneous DEB and BPC. Furthermore, to compare the outcome of BPC in dual approach, transcutaneous procedure and transconjunctival procedure. METHODS: This is a retrospective study reviewing 159 eyelids that underwent mini-invasive DEB with BPC from November 2018 to May 2019, including the technical description and the surgical outcomes. To investigate the efficacy, the pre- and postoperative margin reflex distance 1 ( MRD1) and levator function (LF) corresponding to the different surgical procedures and preoperative severity was analyzed. RESULTS: Statistically, the dual approach group has significant improvement in MRD1 and LF (47 eyelids, p<0.05) corresponding to patients with ptosis of any severity. Under the same tucking amount, dual approach can achieve 1.6±0.7 mm of MRD1 improvement, which is nearly twice the amount compare with transconjunctival approach alone. No revision nor complication noted in the dual approach group by 6-month follow-up. CONCLUSION: The dual approach technique is a method with a wide range of applications, effective, and low revision rate that simultaneously correct blepharoptosis and create a double eyelid. PMID:34930703 | DOI:10.1016/j.bjps.2021.11.062 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Knockdown of circular RNA hsa_circ_0062270 suppresses the progression of melanoma via downregulation of CDC45

xlomafota13 shared this article with you from Inoreader Knockdown of circular RNA hsa_circ_0062270 suppresses the progression of melanoma via downregulation of CDC45 Via Cellular and Molecular Biology Histol Histopathol. 2021 Dec 21:18412. doi: 10.14670/HH-18-412. Online ahead of print. ABSTRACT BACKGROUND: Although systemic therapies for melanoma have been improved, the 5-year survival rate of this aggressive cancer remains poor. It has been shown that hsa_circ_0062270 was upregulated in patients with melanoma. However, the relevant mechanism of hsa_circ_0062270 in the progression of melanoma remains unclear. METHODS: The CCK-8, EdU staining, flow cytometry, and transwell assays were used to determine the viability, proliferation, apoptosis and invasion in melanoma cells. An in vivo animal study was performed finally. RESULTS: The level of hsa_circ_0062270 was significantly upregulated in melanoma cells. In addition, hsa_circ_0062270 knockdown markedly inhibited the viability, proliferation, invasion and promoted the apoptosis of melanoma cells. Cell division cycle protein 45 (CDC45) is the host gene of hsa_circ_0062270 , and downregulation of hsa_circ_0062270 notably decreased the expression of CDC45 in melanoma cells. Rescue assays confirmed that hsa_circ_0062270 regulated the growth of melanoma cells through CDC45. Moreover, RIP analysis showed that hsa_circ_0062270 interacted with RNA-binding protein (RBP) EIF4A3. Furthermore, in vivo study indicated that knockdown of hsa_circ_0062270 inhibited the melanoma tumor growth in vivo. CONCLUSIONS: Downregulation of hsa_circ_0062270 can inhibit the progression of melanoma through downregulation of CDC45. Our findings provide biological mechanisms for the use of hsa_circ_0062270 as a biomarker for melanoma and potential therapeutic target. PMID:34931691 | DOI:10.14670/HH-18-412 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Cerebrospinal Fluid Leak Detection with a Carbon Nanotube-Based Field-Effect Transistor Biosensing Platform

xlomafota13 shared this article with you from Inoreader Cerebrospinal Fluid Leak Detection with a Carbon Nanotube-Based Field-Effect Transistor Biosensing Platform Via leak of cerebrospinal fluid ACS Appl Mater Interfaces. 2021 Dec 21. doi: 10.1021/acsami.1c19120. Online ahead of print. ABSTRACT Cerebrospinal fluid (CSF) leakage may lead to life-threatening complications if not detected promptly. However, gel electrophoresis, the gold-standard test for confirming CSF leakage by detecting beta2-transferrin (β2-Tf), requires 3-6 h and is labor-intensive. We developed a new β2-Tf detection platform for rapid identification of CSF leakage. The three-step design, which includes two steps of affinity chromatography and a rapid sensing step using a semiconductor-enriched single-walled carbon nanotube field-effect transistor (FET) sensor, circumvented the lack of selectivity that antitransferrin antibody exhibits for transferrin isoforms and markedly shortened the detection time. Furthermore, three different sensing configurations for the FET sensor were investigated for obtaining the optimal β2-Tf sensing results. Finally, body fluid (CSF and serum) tests employing our three-step strategy demonstrated high sensitivity, suggesting its potential to be used as a rapid diagnostic tool for CSF leakage. PMID:34932323 | DOI:10.1021/acsami.1c19120 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.