The reparative effects of human adipose-derived mesenchymal stem cells in the chemotherapy damaged thymus.

The reparative effects of human adipose-derived mesenchymal stem cells in the chemotherapy damaged thymus.

Stem Cells Dev. 2018 Dec 04;:

Authors: Zhan Y, Wang L, Liu G, Zhang X, Yang J, Pan Y, Luo J

Abstract
Hematological patients who accept chemotherapy always develop secondary tumor or even die of severe infections. As an important central lymphoid organ, the thymus is frequently damaged during chemotherapy. Previous studies showed that the mesenchymal stem cells (MSCs) can promote the proliferation and repair of epithelial cells in thymus. The purpose of our study is to investigate the reparative effects of human adipose-derived mesenchymal stem cells (hADMSCs) for chemotherapy treated damaged thymus. 80 mice were randomly divided into four groups: normal group, model control group, hADMSCs untreated group and hADMSCs treated group. The mice were injected intraperitoneally with dexamethasone sodium phosphate (Dex 20mg/kg) except the normal group. Then the chemotherapy models were obtained after one week, the treated group were infused intraperitoneally with hADMSCs while the model control group were injected with equal volumes of normal saline. The hADMSCs infusion day was regarded as Day 0. The mice were sacrificed at different time points (Day 3, Day 7, Day 10 and Day 14). The pathologic structure and the function of the thymus, the recovery of T-lymphocyte subpopulation and the proportion of regulatory T (Treg) cells in spleen and peripheral blood were detected. Additionally, we transfected hADMSCs by lentivirus with green fluorescent protein (GFP) to confirm whether they home to thymus and detected the expressions of cytokines that associated with the development of thymus in hADMSCs and thymus. The results of the study showed that the hADMSCs treated group had a more rapid recovery in terms of thymic pathologic structure and function. The hADMSCs could home to the damaged thymus and secrete cytokines that played important roles in repairing damaged thymus. The results indicated that hADMSCs could repair the damaged thymus caused by chemotherapy and improve the immune microenvironment, which may be a potential treatment for hematological patients.

PMID: 30511904 [PubMed - as supplied by publisher]

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