Tetramethylpyrazine inhibits proliferation of colon cancer cells in vitro

paythelady.612 shared this article with you from Inoreader Tetramethylpyrazine inhibits proliferation of colon cancer cells <em>in vitro</em> Μέσω pubmed: "world j clin cases"... World J Clin Cases. 2021 Jun 26;9(18):4542-4552. doi: 10.12998/wjcc.v9.i18.4542. ABSTRACT BACKGROUND: Colon cancer is one of the most common malignancies worldwide, and chemotherapy is a widely used strategy in colon cancer clinical therapy. However, chemotherapy resistance is a major cause of disease recurrence and progression in colon cancer, and thus novel drugs for treatment are urgently needed. Tetramethylpyrazine (TMP), a component of the traditional Chinese medicine Chuanxiong Hort, has been proven to exhibit a beneficial effect in tumors. AIM: To investigate the potential anticancer activity of TMP in colon cancer and its underlying mechanisms. METHODS: Colon cancer cells were incubated with different concentrations of TMP. Cell viability was evaluated by crystal violet staining assay and cell counting kit-8 assay, and cell apoptosis and cell cycle were assessed by flow cytometry. RESULTS: TMP significantly in hibited the proliferation of colon cancer cells in a dose- and time-dependent manner. In addition, flow cytometry revealed that TMP induced cell cycle arrest at the G0/G1 phase. TMP treatment caused early stage apoptosis in SW480 cells, whereas it caused late stage apoptosis in HCT116 cells. CONCLUSION: Our studies demonstrated that TMP inhibits the proliferation of colon cancer cells in a dose- and time-dependent manner by inducing apoptosis and arresting the cell cycle at the G0/G1 phase. Our findings suggest that TMP might serve as a potential novel therapeutic drug in the treatment of human colon cancer. PMID:34222421 | PMC:PMC8223836 | DOI:10.12998/wjcc.v9.i18.4542 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.