MicroRNA‐215‐5p inhibits the proliferation of keratinocytes and alleviates psoriasis‐like inflammation by negatively regulating DYRK1A and its downstream signalling pathways

xlomafota13 shared this article with you from Inoreader MicroRNA‐215‐5p inhibits the proliferation of keratinocytes and alleviates psoriasis‐like inflammation by negatively regulating DYRK1A and its downstream signalling pathways Via Experimental Dermatology Abstract Psoriasis is a chronic inflammatory disease characterized by abnormal hyperproliferation and differentiation. The object of this study is to explore the role of microRNA-215-5p in psoriasis-like inflammation. The expression of miR-215-5p was found to be down-regulated in pro-inflammatory factor-stimulated HaCaT cells and imiquimod (IMQ)-treated skin tissues. Overexpression of miR-215-5p suppressed the proliferation and cell cycle progression of HaCaT cells. Further, miR-215-5p agomir alleviated the disease severity, pathological features and Ki67 positive cells in IMQ-treated mice. Luciferase assay confirmed that miR-215-5p could bind to the 3′UTR of DYRK1A. The in vitro and in vivo results showed that miR-215-5p negatively regulates DYRK1A, which further inhibited EGFR and its downstream signalling pathways, AKT and ERK. Collectively, our results provide evidence that overexpression of miR-215-5p inhibits the proliferation of HaCaT cells and alleviates psoriasis-like inflammati on partly by DYRK1A mediated inhibition of the EGFR signalling pathway. miR-215-5p may serve as a novel small molecule for therapeutic intervention in psoriasis. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.