MITOCHONDRIAL MEMBRANE POTENTIAL, MORPHOLOGY AND ATP PRODUCTION IN MAMMALIAN CELLS EXPOSED TO X-RAYS.

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MITOCHONDRIAL MEMBRANE POTENTIAL, MORPHOLOGY AND ATP PRODUCTION IN MAMMALIAN CELLS EXPOSED TO X-RAYS.

Radiat Prot Dosimetry. 2018 Dec 13;:

Authors: Hamada R, Kaminaga K, Suzuki K, Yokoya A

Abstract
This study aimed to reveal the effect of X-irradiation on mitochondrial function in terms of mitochondrial membrane potential (ΔΨm) and ATP productivity. At the cellular level, ΔΨm was quantified using JC-1, a mitochondrial probe that emits red or green fluorescence at high or low ΔΨm sites, respectively. The fluorescence area was quantified for both colors together relative to the whole-cell area of the same cell. The fluorescence areas versus whole-cell areas varied widely among the irradiated cells depending on the X-ray doses received (6 and 10 Gy) and incubation time, although the relative red area to total mitochondrial area was rather constant. Average ATP concentrations temporarily increased and showed a maximum at 48 h after irradiation for largely G1-arrested cells. These results indicate that an increase of mitochondrial volume per cell, not simply an increase in their active sites, is induced by irradiation, resulting in enhanced ATP production.

PMID: 30544200 [PubMed - as supplied by publisher]

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