Expression of GHR and downstream signaling genes in human adipose tissue - relation to obesity and weight-change.

Expression of GHR and downstream signaling genes in human adipose tissue - relation to obesity and weight-change.

J Clin Endocrinol Metab. 2018 Dec 12;:

Authors: Glad CAM, Svensson PA, Nystrom FH, Jacobson P, Carlsson LMS, Johannsson G, Andersson-Assarsson JC

Abstract
Context: Growth hormone (GH) is a strong regulator of metabolism. In obesity, both GH secretion and adipose tissue GHR gene expression are decreased. More detailed information on the regulation of GHR, STAT3/5 and downstream-regulated genes in human adipose tissue during diet-induced weight loss and weight gain is lacking.
Objective: The aim of this study was to investigate gene expression patterns of GHR and the JAK/STAT pathway (JAK2, STAT3 and STAT5A and STAT5B) in human subcutaneous adipose tissue in relation to energy restriction and overfeeding.
Design, Patients and Interventions: Tissue distribution was analyzed in a dataset generated by RNAseq, containing information on global expression in human tissues. Subcutaneous adipose tissue or adipocyte gene expression (measured by DNA microarrays) was investigated in the following settings: 1) individuals with obesity versus individuals with normal weight, 2) energy restriction and 3) overfeeding.
Results: GHR expression was decreased in subjects with obesity compared to subjects with normal weight (p<0.001), increased in response to energy restriction and decreased in response to overfeeding (p=0.015 and p=0.030, respectively). STAT3 expression was increased in subjects with obesity (p<0.001), decreased during energy restriction and increased during overfeeding (p=0.004 and p=0.006, respectively). STAT3-regulated genes showed an overall picture of being overexpressed in obesity.
Conclusions: This study shows that GHR, STAT3 and STAT3-regulated genes are dynamically, and reciprocally, regulated at the tissue level in response to energy restriction and overfeeding suggesting that GH-signaling is perturbed in obesity.

PMID: 30541116 [PubMed - as supplied by publisher]

from PubMed via alexandrossfakianakis on Inoreader https://ift.tt/2Gf01P2