Analyzing Sinonasal Microbiota of Fungal Rhinosinusitis by Next Generation Sequencing

alexandrossfakianakis shared this article with you from Inoreader Analyzing Sinonasal Microbiota of Fungal Rhinosinusitis by Next Generation Sequencing via Clinical Otolaryngology Abstract Objectives Fungal rhinosinusitis is an inflammatory disease of the nose that may lead to life-threatening complications. This study compared the bacterial and fungal microbiomes between patients with invasive fungal rhinosinusitis (IFRS) and non-invasive fungal rhinosinusitis (NIFRS). Design This was a prospective study including 18 IFRS and NIFRS patients. Fugal and bacterial microbiomes from surgical specimens were sequenced from amplicons of the internal transcribed spacer 1 (ITS1) region and the V3-V4 region of the 16S locus, respectively. Microbiomes were generated using the Illumina MiSeq System 2 x 301 base pair chemistry with a paired–end protocol. Setting Tertiary medical center. Results Targeted metagenomics identified Aspergillus spp. as the predominant fungus in both IFRS and NIFRS patients. Based on phylum and genera level diversity, and abundance differences, significant differences of operational taxonomic units (OTUs) (Fusobacterium, Prevotella, Pseudomonas, Neisseria, and Streptococcus) were more abundant in NIFRS compared with IFRS patients. Conclusions This is the first study to analyze bacterial and fungal microbiomes in patients with IFRS and NIFRS via ITS1 and 16S genomics sequencing. Bacterial microbiomes from patients with IFRS demonstrated dysbiosis (alterations in diversity and abundance) compared to those from patients with NIFRS. View on Web

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