Anti-inflammatory effects and enhancing immune response of freshwater hybrid catfish oil in RAW264.7 cells

xlomafota13 shared this article with you from Inoreader Anti-inflammatory effects and enhancing immune response of freshwater hybrid catfish oil in RAW264.7 cells Via Experimental and therapeutic medicine Exp Ther Med. 2021 Nov;22(5):1223. doi: 10.3892/etm.2021.10657. Epub 2021 Aug 26. ABSTRACT The present study assessed the effect of freshwater hybrid catfish oil (FFO) on the inflammatory status of lipopolysaccharide (LPS)-stimulated RAW264.7 cells and investigated the underlying mechanisms. RAW264.7 cells were supplemented with various concentrations [0.125-2% in 0.5% propylene glycol (v/v)] of FFO with or without LPS (1 µg/ml) for 24 h. Inflammatory cytokines and mediators were quantified using ELISA and reverse transcription-quantitative PCR. The results revealed that FFO treatment inhibited the secretion and mRNA expression of the pro-inflammatory cytokines IL-6, IL-1β, TNF-α. In line with this, FFO suppressed the expression and secretion of the inflammatory mediators cyclooxygenase-2 and prostaglandin E2. FFO also reduced apoptotic body formation and DNA damage. Correspondingly, FFO enhanced the immune response by modulating th e cell cycle regulators p53, cyclin D2 and cyclin E2. Accordingly, FFO may be developed as a nutraceutical product to prevent inflammation. PMID:34603520 | PMC:PMC8453337 | DOI:10.3892/etm.2021.10657 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.