High-level aminoglycoside resistance in Enterococcus faecalis and Enterococcus faecium; as a serious threat in hospitals.

High-level aminoglycoside resistance in Enterococcus faecalis and Enterococcus faecium; as a serious threat in hospitals.

Infect Disord Drug Targets. 2018 Nov 29;:

Authors: Khodabandeh M, Mohammadi M, Abdolsalehi MR, Hasannejad-Bibalan M, Gholami M, Alvandimanesh A, Pournajaf A, Rajabnia R

Abstract
The present work aimed to evaluate the frequency of aminoglycoside-modifying enzymes encoding genes in the E. faecalis and E. faecium and their antibiotic resistance profile. A total of 305 different clinical samples were subjected for identification and antibiotic susceptibility test. High level aminoglycoside resistance was identified by MIC and Kirby Bauer disc diffusion method. The prevalence of aac (6')-Ie-aph (2'')-Ia, aph (3')-IIIa and ant (4')-Ia genes were determined by multiplex- PCR. In total 100 enterococci strains were isolated. The prevalence of E. faecalis and E. faecium isolates were 78% and 22%, respectively. All isolates were susceptible to linezolid. So, all E. faecalis were susceptible to vancomycin but, 36.4% of E. faecium were resistance to it. The prevalence of multiple drug resistance strains were 100% and 67.9% of E. faecium and E. faecalis, respectively. High-level-gentamicin and streptomycin resistant rates were as follows; 26.9% and 73.1% of E. faecalis and 77.3% and 90.1% of E. faecium. The results of the current study showed a high frequency of aac (6')-Ie-aph (2'')-Ia genes among enterococcal isolates. A high rate of resistance to antimicrobials in Enterococcus is obviously problematic, and a novel policy is needed to decrease resistance in these microorganisms.

PMID: 30499420 [PubMed - as supplied by publisher]

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