Graves' Autoantibodies Exhibit Different Stimulating Activities in Cultures of Thyrocytes and Orbital Fibroblasts Not Reflected by Clinical Assays

xlomafota13 shared this article with you from Inoreader Graves' Autoantibodies Exhibit Different Stimulating Activities in Cultures of Thyrocytes and Orbital Fibroblasts Not Reflected by Clinical Assays Via liebertpub1 Thyroid, Ahead of Print. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Migration of Ventriculoperitoneal Shunt to Uterus In A Child: A Case Report

xlomafota13 shared this article with you from Inoreader Migration of Ventriculoperitoneal Shunt to Uterus In A Child: A Case Report Via leak of cerebrospinal fluid J Pediatr Adolesc Gynecol. 2021 Nov 26:S1083-3188(21)00343-0. doi: 10.1016/j.jpag.2021.11.004. Online ahead of print. ABSTRACT BACKGROUND: Ventriculo-peritoneal shunt (VPS) is usually placed inside the peritoneal cavity for cerebrospinal fluid drainage. Rarely, it can migrate to various pelvic visceral organs. Inside the pelvis, the distal end of the shunt can perforate anywhere from the uterus or adnexa to the vulva and migration through the uterus is extremely rare. CASE: A three-and-a-half-year-old girl presented with CSF leak through the vagina following uterine perforation by VPS. The diagnosis was made with an ultrasound. Her symptoms resolved following revision surgery. CONCLUSION: In a patient with a VPS in situ, presenting with a watery fluid leak through vagina, perforation of fornix or uterus must always be kept in mind. Timely diagnosis and intervention can result in the prevention of complications. PMID:34843976 | DOI:10.1016/j.jpag.2021.11.004 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Intraoperative cerebrospinal fluid leak graded by Esposito grade is a predictor for diabetes insipidus after endoscopic endonasal pituitary adenoma resection

xlomafota13 shared this article with you from Inoreader Intraoperative cerebrospinal fluid leak graded by Esposito grade is a predictor for diabetes insipidus after endoscopic endonasal pituitary adenoma resection Via leak of cerebrospinal fluid World Neurosurg. 2021 Nov 26:S1878-8750(21)01802-7. doi: 10.1016/j.wneu.2021.11.090. Online ahead of print. ABSTRACT BACKGROUND: Diabetes insipidus (DI) is a well-known complication of transsphenoidal surgery (TSS). However, the risk factors for DI remain controversial. METHODS: We conducted a retrospective study of the patients who underwent endoscopic TSS for pituitary adenoma at our institution during a 5-year period. The patients were divided into a DI group and a non-DI group. Logistic regression analyses were used to identify risk factors for postoperative DI. In subgroup analysis, the DI group was divided into transient-DI and permanent-DI groups and the perioperative factors were compared between groups. RESULTS: Among 101 patients, 58 patients were classified as non-DI (57.4%) and 43 patients as DI (42.6%) group. Permanent DI occurred in seven patients （6.9%）. In the univariate analyses, statistically significant risk factors were suprasellar extension, tumor functionality and intraoperative cerebrospinal fluid (CSF) leaks by Esposito grade. In the multivariate logistic regression analysis, Esposito grade was the only statistically significant risk factor (P=0.015). The frequency of DI increased as the Esposito grade increased (P=0.0002 for the trend). In subgroup analysis, postoperative nadir sodium concentration was lower in the permanent-DI group (128.1±2.78 mmol/L) than in the transient-DI group (135±1.22 mmol/L) (P=0.035) and the optimal cut-off value was 124.5 mmol/L, with a sensitivity of 57.1% and a specificity of 91.7% (Area Under the Curve=0.76, P=0.034). CONCLUSIONS: Intraoperative CSF leak by Esposito grade is associated with postoperative DI. These data can be applied to help identify high-risk patients who need more-aggressive follow-up and fluid management. PMID:34844009 | DOI:10.1016/j.wneu.2021.11.090 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Comprehensive gene cluster analysis of head and neck squamous cell carcinoma TCGA RNA‐seq data defines B cell immunity‐related genes as a robust survival predictor

xlomafota13 shared this article with you from Inoreader Comprehensive gene cluster analysis of head and neck squamous cell carcinoma TCGA RNA‐seq data defines B cell immunity‐related genes as a robust survival predictor Via Head & Neck Abstract Background The authors aimed to define novel gene expression signatures that are associated with patients' survival with head and neck squamous cell carcinoma (HNSCC). Methods TCGA RNA-seq data were used for gene expression clusters extraction from 499 tumor samples by the "EPIG" method. Tumor samples were then partitioned into lower and higher than median level groups for survival relevant analysis by Kaplan–Meier estimator. Results We found that two gene clusters (_1, _2) are favorably, while two (_3, _4) are unfavorably, associated with patients' survival with HNSCC. Notably, most genes on the top lists of cluster_2 are associated with B cells. A gene expression signature with combined genes from cluster_2 and _4 was further determined to be associated with HNSCC survival rate. Conclusion Our work strongly supported a favorable role of B cells in patients' survival with HNSCC and identified a novel coexpressed gene signature as prognostic biomarker for patients' survival with HNSCC estimation. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Geographic disparities in head and neck cancer survival

xlomafota13 shared this article with you from Inoreader Geographic disparities in head and neck cancer survival in Upstate New York 2011–2019 Via Head & Neck Abstract Purpose To examine the association between distance to care-center and urban–rural residence on 5-year overall survival (OS) from head and neck cancer (HNC). Materials and methods Five-year OS was retrospectively measured from date of initial diagnosis for patients with HNC treated at a single tertiary care center. Distances were calculated based on ZIP code of patient's residence and care center. Multilevel Weibull regression was used to adjust for confounders and identify disparities in 5-year all-cause mortality. Results A total of 670 patients included in study. Multivariable analysis revealed older age or late-stage cancer at diagnosis, and HPV negative status were associated with poorer OS. Patients residing in isolated small rural town (HR = 2.20, p = 0.015) or small rural town (HR = 2.07, p = 0.015) had lower OS. Distance to care center was not associated with OS (HR = 0.996, p = 0.11). Conclusions Greater rurality was associated with poorer OS among HNC patients in Upstate New York. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Achieving negative resection margins in oral cavity cancer with masticator space involvement—Is it feasible? An international collaborative study

xlomafota13 shared this article with you from Inoreader Achieving negative resection margins in oral cavity cancer with masticator space involvement—Is it feasible? An international collaborative study Via Head & Neck Abstract Background Masticator space involvement in oral cavity squamous cell carcinoma (OCSCC) is considered an unresectable disease. Nevertheless, achieving negative resection margins is feasible in limited masticatory space involvement. Materials and Methods A multi-institutional study on OCSCC patients with masticator space invasion who underwent surgical resection. Margin status was assessed according to anatomic tumor involvement of the inframandibular and supra-mandibular notch. Results One-hundred and thirty-two patients met the inclusion criteria. Then, 67 patients (50.8%) were diagnosed with a supra-notch tumor and 65 (49.2%) with an infra-notch disease. Negative margins were more common in the infra-notch group (43.3 vs. 23.1%, p = 0.014), and positive margins were more common in the supra-notch group (41.5 vs. 23.9%, p = 0.041). Multivariable analysis demonstrated that supra-notch tumors had an increased likelihood for involved resection margins (odds ratio = 2.46, p = 0.036). Conclusion OCSCC patients with masticator space involvement are prone for positive surgical margins in tumors extending above the supra-mandibular notch. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Quercetin regulates inflammation, oxidative stress, apoptosis, and mitochondrial structure and function in H9C2 cells by promoting PVT1 expression

xlomafota13 shared this article with you from Inoreader Quercetin regulates inflammation, oxidative stress, apoptosis, and mitochondrial structure and function in H9C2 cells by promoting PVT1 expression Via histochem Acta Histochem. 2021 Nov 26;123(8):151819. doi: 10.1016/j.acthis.2021.151819. Online ahead of print. ABSTRACT OBJECTIVE: To investigate the effect and potential mechanism of quercetin on inflammation, oxidative stress, apoptosis, and mitochondrial structure and function in H9C2 cells. MATERIALS AND METHODS: H9C2 cells were obtained from the Shanghai Institutes for Biological Sciences, Chinese Academy of Science, and randomly divided into six groups: control, model, PVT 1 overexpression (OV), quercetin, OV + quercetin, and NAC groups. The CCK-8 assay was performed to examine cell proliferation. Flow cytometry was used to examine cell apoptosis, cell membrane potential, and ROS levels. The expression of endothelial nitric oxide synthase (eNOS), malondialdehyde (MDA), and superoxide dismutase (SOD) was measured by ELISA and a Biochemical kit. Western blotting was used to determine the levels of p-DRP1 (s637), MFN2, NF-kB, p-NF-kB, IkB, and p-IkB. IL-6, IL-10, TNF-α, and IL-1β mRNA expression was examined by RT-PCR. Electron microscopy was used to observe the structure of mitochondria in H9C2 cells. RESULTS: MDA, p-NF-κB, p-IKB, IL-6, IL-1β, and TNF-α expression levels, and the cell apoptosis rate were significantly higher in the model group than in the control group (P < 0.05). In contrast, the cell proliferation rate and IL-10, SOD, eNOS, and ATP levels were significantly lower in the model group (P < 0.05). Moreover, MDA expression was significantly lower in the OV, quercetin, quercetin + OV, and NAC groups than in the model group (P < 0.05), while SOD, eNOS, and ATP levels were higher. The electron microscopy results showed that PVT1 overexpression or quercetin treatment could inhibit inflammation-induced mitochondrial fission and promote mitochondrial fusion. CONCLUSION: Quercetin promotes the proliferation of H9C2 cells, while inhibiting inflammation, oxidative stress, and cell apoptosis, and alleviating the structural and functional dysfunction of mitochondria. These effects are achieved by promoting PVT1 expression. PMID:34844154 | DOI:10.1016/j.acthis.2021.151819 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.